Classical and Basal Cell Culture Media
NEOGEN Modified Buffered Peptone Water with Pyruvate, 500 g
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
NEOGEN Perfringens Agar Base (TSC), 500 g
This item has a minimum order quantity of 2 per supplier requirements
Perfringens Agar Base (TSC) is used with selective and differential supplements for the recovery of Clostridium perfringens, and is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to ISO 14189:2013, Perfringens Agar Base (TSC) is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.For more information visit https://www.neogen.com/solutions/Microbiology/perfringens-agar-base
NEOGEN Harlequin Cronobacter Isolation Agar, 500 g
Chromogenic Cronobacter Isolation Agar (CCI) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Tryptic digest of casein and yeast extract provide a source of nitrogen and essential vitamins. The high content of sodium chloride and the presence of sodium desoxycholate produce a selective and specific medium.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-cronobacter-isolation-agar
NEOGEN DNase Agar, 500 g
DNase Agar is used for the differentiation of microorganisms on the basis of deoxyribonuclease activity and is not intended for use in the diagnosis of disease or other conditions in humans.In 1956, Weckman and Catlin showed a correlation between increased DNase activity of Staphylococcus aureus and positive coagulase activity. Their research suggested DNase activity could be used to identify potentially pathogenic staphylococci. DiSalvo confirmed their results by obtaining excellent correlation between coagulase and DNase activity of staphylococci isolated from clinical specimens. Jeffries, Holtman, and Guse incorporated DNA in agar to study DNase production by bacteria and fungi. Polymerized DNA precipitates in the presence of 1N HCl, creating an opaque medium. Organisms that degrade DNA produce a clear zone around an inoculum streak.For more information visit https://www.neogen.com/solutions/Microbiology/dnase-agar
NEOGEN Bacteriological Peptone, 500 g
Bacteriological Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.An economical source of nutrients provided by a balanced mixture of meat peptones and tryptone. The growth requirements of most non-fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.For more information visit https://www.neogen.com/solutions/Microbiology/bacteriological-peptone
NEOGEN Harlequin E. coli/Coliform Agar, 500 g
E. coli / Coliform Agar is for the simultaneous enumeration of Escherichia coli and coliforms in food and environmental samples and is not intended for use in the diagnosis of disease or other conditions in humans.This dual chromogenic substrate medium makes the different colony types easy to distinguish allowing rapid counting of both E. coli and coliforms on a single agar.Based upon the formulation of Tryptone Bile Agar, the medium has been modified by the addition of two chromogenic substrates, one to detect the ß-glucuronidase enzyme (X- glucuronide) and another to detect the ß-galactosidase enzyme (magenta-ß-gal). Typical E. coli strains possess both enzymes but only cleave the X-glucuronide substrate, thereby producing blue-green colonies. Typical coliforms, however, possess only the ß-galactosidase enzyme and produce rose-pink colonies.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-e-coli-coliform-agar
NEOGEN Alkaline Saline Peptone Water (ASPW) (ISO), 500 g
Alkaline Saline Peptone Water is a medium for the enrichment of Vibrio spp. from food and water samples according to ISO 21872-1:2017 and ISO 21872-2:2007, and is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Shread, Donovan & Lee as an enrichment broth for Aeromonas spp. and identified by Cruickshank as an effective medium for the enrichment of Vibrio spp., Alkaline Saline Peptone Water uses elevated pH and salt levels to provide a favorable environment for the enrichment of Vibrio spp.Peptone provides essential vitamins, minerals, amino acids & nitrogen for growth requirements. Sodium chloride provides essential electrolytes for maintenance of the osmotic balance.Sub-culture onto Thiosulfate Citrate Bile Salts Sucrose (TCBS) Agar (NCM0052) will be required.For more information visit https://www.neogen.com/solutions/Microbiology/alkaline-saline-peptone-water-iso
NEOGEN Sabouraud Dextrose Agar, Emmons, 500 g
Sabouraud Dextrose Agar, Emmons is used for the cultivation of fungi in a laboratory setting. Sabouraud Dextrose Agar, Emmons is not intended for use in the diagnosis of disease or other conditions in humans. Sabouraud Dextrose Agar (SDA), Emmons is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. Emmons modified the formula by adjusting the pH and reducing the dextrose concentration. This neutral pH enhances recovery of some pathogenic fungi. Sabouraud Dextrose Agar, Emmons is an excellent basal medium, and antibiotics and inhibitors may be added for the selective cultivation and isolation of various microorganisms.For more information visit https://www.neogen.com/solutions/Microbiology/sabouraud-dextrose-agar-emmons
NEOGEN Universal Beer Agar, 500 g
Universal Beer Agar is used for the cultivation of bacteria and yeasts encountered in the brewing industry in a laboratory setting. Universal Beer Agar is not intended for use in the diagnosis of disease or other conditions in humans. Universal Beer Agar was developed by Kozulis and Page and used as a basal medium supplemented with beer. The developers compared this formula with other media commonly used in breweries for detecting microbial contamination. The characteristics of Universal Beer Agar are closer to natural environmental conditions found in a typical brewery. Universal Beer Agar supports the growth of more lactic acid bacteria and yields larger colonies in a shorter time than traditional brewer’s media. Supplementing this medium with beer has produced a selective environment for organisms that are adapted to existent conditions in the brewery.For more information visit https://www.neogen.com/solutions/Microbiology/universal-beer-agar
NEOGEN Bile Salts No. 3, 500 g
Bile Salts No. 3 is a mixture of bile salts for use in preparing microbiological culture media in a laboratory setting. Several media containing Bile Salts No. 3 are recommended in standard methods for multiple applications. Bile Salts No. 3 is not intended for use in the diagnosis of disease or other conditions in humans.Bile Salts No. 3 is a refined bile salts mixture, comprising mainly sodium cholate and sodium desoxycholate. Its primary use is as a selective agent for the isolation of Gram-negative micro-organisms, inhibiting Gram-positive cocci. Bile Salts No. 3 is prepared especially for use in MacConkey Agar and Violet Red Bile Agar. This fraction of bile is highly active allowing maximum selection of organisms of enteric origin at relatively low concentrations (0.15%) and is soluble in distilled or deionized water and neutral to slightly alkaline in pH.For more information visit https://www.neogen.com/solutions/Microbiology/bile-salts-number-3
NEOGEN D/E Neutralizing Broth without Tween, 500 g
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans.D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.For more information visit https://www.neogen.com/solutions/Microbiology/de-neutralizing-broth-without-tween
NEOGEN m-Enterococcus Agar, 500 g
m-Enterococcus Agar is used for the selective isolation and enumeration of enterococci by membrane filtration in a laboratory setting. m-Enterococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-Enterococcus Agar was first described by Slanetz et al. for the enumeration of enterococci by the membrane filtration technique. In 1957, Slanetz and Bartley modified this medium by adding triphenyl tetrazolium chloride (TTC). Increased recovery and larger colonies were obtained by incubating the inoculated membranes on the agar surface instead of on pads saturated with liquid medium. The membrane filtration method is simple to perform, does not require confirmation, and permits a direct count of enterococci in 48 hours. m-Enterococcus Agar is also referred to as m-Azide Agar.For more information visit https://www.neogen.com/solutions/Microbiology/m-enterococcus-agar
NEOGEN Brilliant Green Agar with Sulfapyridine, 500 g
Brilliant Green Agar w/ Sulfapyridine is used for the selective enrichment of Salmonella spp. in a laboratory setting. Brilliant Green Agar w/ Sulfapyridine is not intended for use in the diagnosis of disease or other conditions in humans.Brilliant Green Agar was first described by Kristensen et al and later modified by Kauffmann. The outstanding selectivity of this medium permits the use of moderately heavy inocula evenly distributed over the surface. The addition of sulfonamides into Brilliant Green Agar further inhibits Escherichia coli and Proteus spp. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-with-sulfapyridine
NEOGEN Maximum Recovery Diluent (Tryptone Salt Broth), 500 g
Maximum Recovery Diluent (Tryptone Salt Broth) is used as an isotonic diluent for maximum recovery of microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. An osmotically controlled solution which is an alternative to, and a replacement for, 1/4 strength Ringer’s Tablets (NCM0191K). The presence of a low level of peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringer’s Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent (1-2 hours). This formula is recommended by ISO 6887-1:2017 For more information visit https://www.neogen.com/solutions/Microbiology/maximum-recovery-diluent-tryptone-salt-broth
NEOGEN Tryptose No. 2, 500 g
Tryptose No. 2 is a special blend of peptones developed for use in the production of Foot and Mouth vaccine, and is not intended for use in the diagnosis of disease or other conditions in humans.A special blend of peptones originally developed for use in the production of Foot and Mouth vaccine. Other peptone blends are also available within the catalogue, some of which have been evaluated to demonstrate low cell toxicity and the ability to enhance the growth of cell lines (eg NCM0264).For more information visit https://www.neogen.com/solutions/Microbiology/tryptose-number-2